Genotypic resistance testing in HIV by arrayed primer extension

Genotyping with a 198 Mutation Arrayed Primer Extension Array for Hereditary Hearing Loss: Assessment of Its Diagnostic Value for Medical Practice

Molecular diagnostic testing of individuals with congenital sensorineural hearing loss typically begins with DNA sequencing of the GJB2 gene. If the cause of the hearing loss is not identified in GJB2, additional testing can be ordered. However, the step-wise analysis of several genes often results in a protracted diagnostic process. The more comprehensive Hereditary Hearing Loss Arrayed Primer...

Use of genotypic resistance testing to guide hiv therapy: clinical impact and cost-effectiveness.

BACKGROUND Genotypic sequencing for drug-resistant strains of HIV can guide the choice of antiretroviral therapy. OBJECTIVE To assess the cost-effectiveness of genotypic resistance testing for patients acquiring drug resistance through failed treatment (secondary resistance) and those infected with resistant virus (primary resistance). DESIGN Cost-effectiveness analysis with an HIV simulati...

Author's response to reviews Title: Robust SNP genotyping by multiplex PCR and arrayed primer extension Authors:

Evaluating the arrayed primer extension resequencing assay of TP53 tumor suppressor gene.

Identification of mutations in the tumor suppressor gene TP53 has implications for the molecular epidemiology and for the molecular pathology of human cancer. We have developed and evaluated an arrayed primer extension assay for covering both strands of a region of the coding sequence containing more than 95% of the mutations described so far in TP53. On average, 97.5% of the arrayed TP53 gene ...

The application of artificial neural networks for phenotypic drug resistance prediction: evaluation and comparison with other interpretation systems.

Although phenotypic resistance testing provides more direct measurement of antiretroviral drug resistance than genotypic testing, it is costly and time-consuming. However, genotypic resistance testing has the advantages of being simpler and more accessible, and it might be possible to use the data obtained for predicting quantitative drug susceptibility to interpret complex mutation combination...